RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Cte dIvoire

The application of bacteriophages biocontrol requires the formulation genetically distinct in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered cheap, reproducible, and readily applicable tool detecting diversity compared to other molecular techniques such as whole-genome sequencing. We used this study RAPD-PCR technique assess genetic 28 infecting Pseudomonas aeruginosa Staphylococcus aureus. According their RAPD profiles, isolated phages were grouped into 2 main clusters which included from same host. typing by newly was useful bypassing previous sequencing analysis. These lytic against P. S. aureus could potentially be cocktail for these clinically industrially relevant bacteria.   Key words: Phages, diversity, PCR, aeruginosa,